The TCRexpress clone screening kit is designed for quick and easy screening of TCR clones in the established human monoclonal T cells, such as the CTL clones or T cell lines
Two reagent kits designed to quickly screen T cell receptor V beta usage are available from Wisbiomed.
These kits are the expansion of its TCRexpress clonality assay kits and TCRexpress quantification kits.
The TCRexpress clone screening kit is designed for quick and easy screening of TCR clones in the established human monoclonal T cells, such as the CTL clones or T cell lines.
The assay system is based on the fact that the established monoclonal T cells express only one specific Vb chain while polyclonal T cells express multiple Vb families.
The uniquely designed primer sets are composed of one Vb, one CB and internal controls, 3'Control and 5'Control genes, for PCR amplification.
The primers and reagents are provided in preloaded chimney-well PCR plates to reduce the potential risk of contamination.
The system is simple in design, easy to use and able to produce rapid, reliable results.
With the included fine-tuned protocol, the kit provides a off shelf solution.
No extensive training is required.
Moreover, a band of interest from a particular screened clone can be cut from the gel and purified for direct sequencing of the CDR3 region.
Assay requires RNA extracted from one million cells.
Most assays can be completed within one day.
There are two products, the hTCRexpress clone screening kit for human samples and the mTCRexpress clone screening kit for mouse samples.
Kits come with all reagents needed to complete the assay.
