The labelling of proteins and antibodies with fluorescent dyes can play a crucial role in flow cytometry, immunohistochemistry and fluorescent in-situ hybridisation techniques
Incorporating its high performance HiLyte Fluor dyes in an a ready-to-use kit, AnaSpec has introduce its new line of AnaTag protein labelling kits as a convenient method for labelling proteins with AnaSpec dyes.
AnaTag protein labelling kits are designed for fast and convenient kit labelling of primary and secondary antibodies.
Containing all essential components for conjugation reaction and purification of dye-protein conjugates, AnaSpec notes that labeling time can be as short as 30 minutes.
Available in two reaction sizes: micro-scale (200ug x three reactions or 100ug x five reactions) and larger scale (5mg x three reactions).
Utilising both AnaSpec's proprietary HiLyte Fluor dyes as well as classic dyes, AnaTag protein labelling spans the spectrum from blue to far red and NIR.
The succinimidyl ester (SE) reactive form of the labelling dye reacts with the amine group on the protein and forms stable carboxamide bonds which are identical to and as stable as the natural peptide bond.
These dye-protein conjugates are very stable and are suitable for immunofluorescent staining, fluorescence in situ hybridisation, flow cytometry and other biological applications.
