Available from Eppendorf is a new application note that describes how electroporation is used for the transfection of Jurkat cells with small interfering RNA (siRNA)
The efficient transfer of siRNA into cells is a prerequisite for a successful gene silencing approach.
This protocol describes how to determine the best electroporation conditions (voltage and pulse duration) and the optimum concentration of siRNA.
A high transfection rate and minimum cell damage result in a silencing efficiency of 85%.
For the electroporation experiments, the Eppendorf Multiporator and Eppendorf hypoosmolar electroporation buffer were used.
The Multiporator applies electronically regulated microsecond pulses (high cell survival rate), in combination with the hypoosmolar buffer a high transfection yield is achieved.
The application note, 'Optimising electroporation parameters for effective gene silencing in Jurkat cells' (Note 137), can be downloaded from the Eppendorf website.
