Thermo says that targetted protein quantitation in complex matrices can be routinely achieved by high-throughput highly selective reaction monitoring (H-SRM) using the TSQ Quantum Ultra
By reducing matrix interference without significant signal loss, the TSQ Quantum Ultra provides enhanced assay sensitivity, selectivity and robustness enabling researchers to accurately quantify more proteins, more reliably.
This system will be displayed at the 55th Annual ASMS conference on mass spectrometry.
H-SRM scans are fast (two milliseconds) and sensitive, allowing practical monitoring and quantitation of hundreds of proteins per second during LC-MS/MS analysis.
With this speed, there is no need for complicated MRM scheduling methods, all proteins are monitored during the entire analysis.
In addition, the triple-quadrupole based method produces high-quality confirmation of peptide structure, as targeted proteins are identified by automatic data dependent MS/MS scans.
"The TSQ Quantum instrument is the perfect system for protein quantitation in complex matrices", states Iain Mylchreest, vice president of Thermo Fisher's life sciences mass spectrometry.
"We have convincingly demonstrated that the combination of fast H-SRM scanning, with peptide confirmation by MS/MS, allows accurate quantitation for targeted biomarker verification or protein pathway analysis.
"In addition, Aqua labelled internal standards will increase assay accuracy and precision and allow for true, validated protein assays".
The TSQ Quantum triple quadrupole system is the only instrument that enables highly selective reaction monitoring (H-SRM) performance, allowing complex samples to be analysed quickly and efficiently.
HyperQuad quadrupoles yield higher ion transmission, minimising false positives and maximising sensitivity.
The TSQ Quantum features a unique heated ESI probe with dual desolvation zone technology that further optimises performance.
