A highly sensitive SensoLyte 520 beta-secretase assay kit based on a HiLyte Fluor 488/QXL 520 Fret substrate has been developed by AnaSpec
At the 2007 Experimental Biology conference in Washington, DC, AnaSpec prepared a technical poster entitled 'A sensitive fluorimetric assay for detection of beta-secretase activity using a novel Fret peptide substrate'.
The poster documented the following findings.
The longer excitation and emission wavelengths of HiLyte Fluor488 minimize the interference from autofluorescence and absorbance of test compounds.
This SensoLyte 520 b-secretase assay kit is capable of continuous, homogeneous monitoring of the enzymatic reaction.
IC50 value for an inhibitor determined with SensoLyte 520 b-secretase assay kit were consistent with published data.
