Invitrogen launches Molecular Probes Qdot nanocrystal conjugated primary antibodies for multicolour flow cytometry, and Click-iT glycoprotein profiling reagents based on novel 'click' chemistry
Molecular Probes Qdot nanocrystal conjugated primary antibodies for use in multicolour flow cytometry are directly labelled fluorescent reagents which allow cell biologists to use an array of distinct colours to track multiple parameters within a single experiment.
When excited by a single light source, Qdot nanocrystals emit in a range of brilliant distinct colors with long-term photostability, offering researchers greater flexibility and precision in designing multicolor flow cytometry panels.
Click-iT glycoprotein profiling reagents are said to be the first commercially available glycoprotein analysis tools based on click chemistry.
This powerful approach provides unprecedented selectivity, sensitivity, and versatility in glycoprotein characterisation, which overcomes the limitations of existing lectin-based or antibody-based techniques.
The Click-iT methodology first involves a labeling step, in which a very small bioorthogonal moiety, or handle is either metabolically or enzymatically incorporated into the glycan structure.
Next, in the detection step, a fluorescent label or biotin is selectively attached to the modified glycoprotein - a reaction typically completed in less than an hour.
This simple, two-step procedure is highly efficient and produces a very stable covalent bond - one capable of surviving mass spectrometry ionization - and compatible with multiplexed proteomics applications using 1- or 2D gel electrophoresis or western blot analysis to detect total proteins, total glycoproteins or total phosphoproteins simultaneously with the Click-iT labelled glycoproteins.
