With excitation wavelengths of 555, 647 and 750nm, the HiLytePlus series is optimised for long wavelength biomarker labelling
AnaSpec says its newly-released HiLytePlus series of fluorescent dyes demonstrate even greater brightness than its HiLyte Fluor line, and represents the next generation in the company's line of high performance fluorescent dyes.
R+D manager Rich Meyer noted: "Our original HiLyte Fluor series was already returning superior performance results in comparison to Cy and similar dyes.
"With the introduction of our HiLytePlus series, we are looking to distance ourselves even further in terms of performance.
"By maintaining a price point that is significantly more cost effective than our major competitors, we believe that our HiLytePlus series will prove to be extremely competitive in the high performance biomarker labelling market".
The HiLytePlus series dyes are available individually and in AnaTag labelling kits, labelled secondary antibodies, and labelled streptavidin.
The individual dyes are available in different reactive forms - acid, amine, C2 maleimide, hydrazide, and succinimidyl esters (SE).
Goat anti-rabbit antibody was labelled with a mole ratio of 8:1 of each of the indicated dyes in to give a degree of substitution of 3 (+/-8%).
Solutions of rabbit IgG were added to the wells of high-binding 96-well plates, which were then blocked with BSA.
A solution of the labelled antibodies was added and the plates, after incubation and washing, were read at the optimal excitation and emission wavelengths.
