KapaHiFi DNA polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends
GR announces the launch of KapaHiFi DNA polymerase from Kapa Biosystems.
KapaHiFi is a second generation DNA polymerase engineered for extreme fidelity and robustness.
The result is industry-leading performance providing superior fidelity, robust performance across a wide range of difficult templates, long range with amplification of targets greater than 18 kb and high speed with reduced reaction times of up to 75%.
Processivity is defined as the number of nucleotides incorporated by the polymerase per binding event, and is a major determinant of extension speed.
In contrast to fusion technologies that attach DNA binding proteins to Pfu-like polymerases to improve processivity, KapaHiFi DNA polymerase has been engineered to have an increased affinity for DNA without the need for accessory protein domains.
The intrinsic high processivity of KapaHiFi results in significant improvements in yield, speed, target length, and the ability to amplify difficult templates.
Typical proofreading polymerases require extension times of 1-2 minutes per kilobase.
KapaHiFi DNA polymerase requires extension times of just 30 seconds per kilobase, providing the potential to save up to 75% of total reaction time.
KapaHiFi also possesses a strong 3'-5' exonuclease-dependent proofreading activity resulting in superior accuracy.
