MiScript Controls are a collection of six highly validated primer assays which enable normalisation of real-time PCR results in miRNA quantification studies
Qiagen says its miScript Controls are specific for several small nucleolar RNAs and small nuclear RNAs (snoRNAs or snRNAs).
Due to thoughtful assay design, each miScript Control is effective in human, mouse, and rat, enabling miRNA experiments in all three species.
For accurate, reproducible real-time PCR analysis of miRNA expression, it is necessary to normalize measurements by comparing results from the target miRNA to those from a suitable endogenous reference RNA.
This corrects for experimental variations which could otherwise lead to inaccurate quantification, and allows results from different experiments to be compared directly.
miScript Controls have high amplification efficiencies, are optimized to work under standard assay conditions, and target small RNAs which are constantly expressed in multiple different sample types.
These features make miScript Controls ideal for data normalisation.
miScript Controls, in combination with the miScript System for Sybr Green-based real-time PCR, enable accurate quantification of hundreds of miRNAs from a single cDNA synthesis reaction.
