GE Healthcare introduces a protein labelling trial kit for 2D fluorescence difference gel electrophoresis (2D Dige), offering a quick, easy and low-cost opportunity for scientists new to the system
The Dige trial pack contains 2nmol each of the three CyDye Dige Fluor minimal dyes Cy2, Cy3 and Cy5, sufficient to run five gels (ten protein samples plus internal standards), and a free one month trial license of DeCyder 2D differential analysis software, the dedicated image analysis software for the Ettan Dige system.
The 2D Dige system offers significant cost and time-saving benefits in comparison with classical 2D electrophoresis.
Protein mixtures are labelled prior to electrophoresis with size and charge-matched, spectrally resolvable CyDye fluors, allowing the simultaneous separation and comparative analysis of up to three samples on a single 2D gel.
Following 2D electrophoresis, the results may be scanned with the Typhoon variable mode imager or Ettan Dige imager.
DeCyder differential analysis software automatically locates and analyses protein spots, assigning statistical confidence to every difference between the samples.
As the system ensures that each protein spot has its own internal standard, gel variation issues are eliminated, significantly increasing accuracy and reproducibility.
