Higher emission wavelength allows the SensoLyte 570 to offer greater sensitivity in detecting enzyme activities with minimal interference from autofluorescence of cell components and test compounds
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components.
The importance of MMPs in tumour development and invasion as well as other diseases is well known.
AnaSpec has introduced the SensoLyte 570 generic MMP assay kit, claiming the industry's highest emission wavelength for MMP detection.
This kit is ideal for detecting generic MMP activity in biological samples or for high throughput screening of MMP inducers and inhibitors using purified MMPs.
The SensoLyte 570 generic MMP assay kit is optimised to detect the activity of a variety of MMPs, including MMP-1, 2, 7, 8, 9, 10, 13, and 14.
This kit uses a Fret peptide substrate labeled with 5-Tamra (fluorophore) and QXL 570 (quencher) for continuous measurement of enzyme activities.
In an intact Fret peptide, the fluorescence of 5-Tamra is quenched by QXL 570.
Upon the cleavage of the Fret peptide by MMPs, the fluorescence of 5-Tamra is recovered, and can be continuously monitored at excitation/emission = 540nm/575nm.
The assays are performed in a convenient 96-well format.
