Qiagenes Expression Kits, a set of ready-cloned plasmids with synthetic genes covering the entire human genome, are now available for use in insect or mammalian cells.
These kits are Qiagen's and collaboration partner Geneart's response to researchers' desires for alternatives to protein production using E.coli cells.
Qiagenes Expression Kits use synthetic complementary DNA (cDNA) with a human genome sequence optimised for expression in E.coli, insect or mammalian cells.
This optimised sequence increases protein yields by as much as 50-fold and produces expression success rates as high as 90 per cent.
Qiagenes Expression Kits include ready-to-go vectors, but are also flexible enough to express only parts of a gene, or to use N-terminal His-tag if needed.
They are compatible with Qiagen protein kits, including all Ni-nta products for His-tag-based purification.