Anaspec has introduced its latest collection of streptavidins labelled with its Hilyte Fluor and Hilyte Plus dyes that promise brighter fluorescence and better photostability compared to other dyes.
Both avidin and streptavidin show strong affinity to biotin.
The dissociation constant (Kd) of the non-covalent interaction between streptavidin and biotin is on the order of 10-15M.
The streptavidin-biotin complex is also highly stable over a wide range of temperatures and pH.
As a result, the streptavidin-biotin interaction has been extensively used in molecular biology, immunology and cellular assays.
Streptavidins labelled with fluorescent dyes are widely used for immunofluorescent staining, fluorescence activated cell sorting, in-situ hybridisation and other biological applications.
Anaspec's Hilyte Fluor labelled streptavidin sampler kits, available in 2 sizes, contain streptavidins labelled individually with Hilyte Fluor.
Prepared at optimal fluorophore to streptavidin labelling ratio, these Hilyte Fluor streptavidin conjugates exhibit high fluorescence signal and uncompromised biotin binding capability.
Every lot has been validated with immunofluorescence staining.
