Anaspec has released the improved Anatag APC, B-PE and R-PE labelling kits, which contain all the reagents needed for optimised antibody labelling.
The phycobiliproteins, modified by SMCC, result in the creation of SH-reactive moieties, which can react with the thiol groups of target antibodies.
Since no additional activation is required, the conjugation process is simple and easy.
Phycobiliproteins are soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae.
Allophycocyanin (APC) is a sensitive, near-infrared fluorescent tracer with a high quantum yield (Ex/Em = 650nm/660nm).
B-Phycoerythrin (B-PE), a fluorescent protein from the phycobiliprotein family, has a primary absorption peak at 545nm with a secondary peak at 563nm and maximum emission at 578nm.
R-Phycoerythrin (R-PE), made of a, b and g subunits and present as (ab)6g, has broad absorption bands with peaks at 565nm (eM =1.96 X 106 M-1cm-1), 498 (eM =1.53 X 106 M-1cm-1) and 539nm (eM =1.62 X 106 M-1cm-1).
These phycobiliproteins are more sensitive and photostable than conventional organic fluorophores.
APC, B-PE or R-PE labelled antibodies are used in applications such as flow cytometry and immunofluorescence.
Anatag kits provide all the reagents and purification column needed to label up to 1mg of antibody.
A detailed step-by-step protocol is also included.
APC, B-PE or R-PE and SMCC activated derivatives of these proteins can be purchased separately.
