Worthington's Hepatocyte Isolation System contains a pre-optimised combination of enzymes to minimise the lot-to-lot variation and improve the quality of the isolated hepatocytes.
Worthington use-tests each lot by isolating hepatocytes from adult rats; this assures performance, reliability and consistent yield of viable cells.
Each kit includes single-use enzyme vials, balanced salt solution, buffer, culture media and an optimised protocol for five separate adult rat liver perfusions.
A modified protocol for mouse applications is also available.
The method is based on that described by M Berry, modified by P Seglen and optimised in conjunction with several collaborating researchers.
