Anaspec has released the Clearpoint collection of beta-amyloid peptides.
Clearpoint peptides are heavy or stable isotope labelled peptides and amino acids.
These peptides can be used for the quantitative analysis of b-Amyloid peptides in biological fluids using mass spectrometry (MS).
The inclusion of internal heavy isotope labelled peptide standards makes mass spectrometry an absolute quantitation (AQUA) method for the peptide of interest.
B-Amyloid (1-40) and (1-42) peptides have been used as biomarkers of Alzheimer's disease (AD).
Historically, ELISA assays have been used in the quantitation of these peptides.
However, technical challenges, such as the tendency of b-Amyloid (1-40) and (1-42) peptides to self-aggregate, undergo post-translational modifications or bind to other proteins, result in inaccuracies in quantitation when using ELISA.
The use of [15N]-labelled b-Amyloid peptides as internal standards using immunoaffinity- purified CSF.1 has been reported.
