Anaspec has introduced the Hilyte Fluor 594 (Ex/Em=593/616 nm), a bright and red fluorescent probe.
Hilyte Fluor dyes are a series of high-performance fluorescent labelling dyes that span the visible spectrum.
These dyes yield maximum brightness when conjugated with macromolecules.
They are available in a variety of reactive forms, such as amine-reactive, thiol-reactive and amine-containing derivatives, allowing easy conjugation to biopolymers.
Hilyte Fluor 594 has spectral characteristics similar to those of Texas Red, with excitation and emission wavelength at 593/615nm in PBS buffer (pH 7.4) and 596/617nm when conjugated to macromolecules.
It has a high extinction coefficient (80,000 M-1cm-1) and a low correction factor (0.17).
The fluorescence quantum yield of Hilyte Fluor 594 is 0.9 in aqueous solution.
Hilyte Fluor 594 conjugated streptavidin provides high fluorescence intensity and low background as validated in immunofluorescence staining of mammalian cells.
Bioconjugates conjugated to Hilyte Fluor 594 exhibit little spectral overlap with green-fluorescent conjugates and can be efficiently excited by 568nm line of Ar-Kr laser and by the 594nm line of orange He-Ne laser.
Hilyte Fluor 594 conjugated proteins have been optimised in a specific fluorophore / protein labelling ratio to ensure a high fluorescent signal and uncompromised protein functions.
The Hilyte Fluor 594-protein conjugates are suitable for immunofluorescent staining, in situ hybridisation, flow cytometry and other biological applications.
