Anaspec has released sensitive assay kits for detecting alkaline phosphatase activities.
The Sensolyte Luminescent Alkaline Phosphatase and Sensolyte Luminescent Alkaline Phosphatase Elisa assay kits contain a chemiluminescent substrate capable of detecting enzyme activities in solutions and in biological samples at femtogram level.
The luminescent substrate supplied in the kits provides high signal and low background, wide dynamic range results and good reproducibility.
The kits provide sufficient reagents to perform 500 assays in a 96-well plate format.
Phosphatases play a key role in cell signal transduction, differentiation and proliferation with alkaline phosphatase catalysing the dephosphorylation of biomolecules in an alkaline environment.
Alkaline phosphatase is present in all human tissues, particularly in the liver, bile duct, kidney and placenta.
The level and activity of alkaline phosphatase correlates with many physiological and pathological events, such as bone development and bone-related disease, renal disease, pregnancy and drug intoxication.
Alkaline phosphatase is widely used as a reporter for gene expression and for Elisa.
