The ATP Biomass Kit HS from BioThema, available from Labtech, is intended for determination of total ATP (extra-plus intracellular) in samples containing living cells.
All living cells contain ATP where it plays the role of energy currency between different cellular processes.
The intra-cellular concentration of ATP is carefully regulated to similar levels in all types of cells.
ATP is therefore a good estimate of the total intracellular volume.
The sensitivity of this kit allows determination of low ATP levels in a variety of circumstances provided extracellular ATP is not a problem: bacteriological control of liquids (for example, drinking water, process water, beverages and beer); pharmaceuticals and cosmetics; cell adhesion to surfaces; quality assurance of laundry; bacterial growth; and antibiotic effects on bacteria.
The kit contains reagents for 150-375 assays (each vial of ATP Reagent HS is sufficient for 25 assays in a tube luminometer and 62.5 assays in a microplate luminometer).
Any tube or microplate luminometer can be used.
Tube luminometers are about one order of magnitude more sensitive.
If a microplate luminometer is used it should have minimum two dispensers and should be able to mix the microplate.
