Anaspec has introduced a line of b-amyloid peptides labelled with biotin (N or C-terminal biotin labels) and N-terminal biotin-labelled b-amyloid peptides with a fluorescein label on the C-terminus.
The peptides have already been used in different applications.
Nelson, TJ, and Alkon, DL, used biotin-LC-b-amyloid-(1 - 42) in Ab-proteins binding studies.
Ab-proteins complex were precipitated with avidin-agarose and western blots run.
A paper by Bohrmann, B et al reported the use of biotin-Ab(1-40)0) or biotin-Ab (1-42) for Ab polymerisation studies.
Biotin-Ab (1-40) or biotin-Ab (1-42) peptides were incubated in Ab (1-40) or Ab (1-42) coated plates, and streptavidin-peroxidase was added.
Colour development was done using tetramethyl-benzidin, a chromogenic substrate for peroxidase.
In the same paper, biotin-Ab (1-40) incorporation into preformed Ab (1-42) fibrils was reported.
Biotin-Ab (1-40) was added to a low concentration of Ab (1-42) fibrils and stained with anti-biotin Ig labelled with colloidal gold and negatively stained with uranyl acetate.
A paper by Liu, Y et al shows the use of biotinylated fibrillar Ab (1-42) in electron microscopy.
Using what they called 'FAbB', fluorescein beta-amyloid (1-40)-Lys(LC-biotin), Leissring, MA et al performed proteolysis measurement using fluorescence polarisation (FP) technique.
