Epigenetics researchers can now detect changes in methylation status in a real-time format using high-resolution melting (HRM) analysis.
Qiagen's Epitect HRM PCR kit provides a master mix format for the detection of changes in the CpG methylation status of bisulfite converted DNA.
The kit is designed to run on all real-time cyclers, including the Rotor-Gene Q, Qiagen's real-time PCR cycler, which provides a highly specific melting curve.
HRM technology enables rapid characterisation of DNA samples based on their melting behaviour following PCR amplification.
'HRM is ideal for screening larger numbers of samples and for the detection of changes in the methylation degree of specific DNA regions,' said Gerald Schock, Qiagen's senior global product manager, Epigenetics.
'It can be used to identify regions of interest for epigenetic researchers and then be further analysed by methods such as Pyrosequencing, which provides quantitative sequence data,' he added.
Epigenetics focuses on differences in gene regulation and expression resulting from DNA methylation.
'The Epitect portfolio and Qiagen automation offer customers a set of standardised tools optimised for epigenetics research,' said Schock.
While HRM gives researchers the ability to detect changes in the degree of methylation and compare differences gene to gene, some research may require a closer look at the methylated region.
Qiagen's complete workflow allows researchers to continue looking at the specific sequence of those regions through Pyrosequencing.
The instrument systems in Qiagen's Pyromark portfolio, such as the newly launched Pyromark Q24, use proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic testing and epigenetic methylation analysis.
The Pyromark instruments combine both quantification and sequencing for highly reliable results in minutes.
The instruments also are highly suited for the discovery and analysis of allelic mutations as well as analysis and quantification of consecutive CpG sites.
All Pyromark systems provide a sequencing instrument, sample preparation workstation, and reagents and controls, as well as comprehensive and flexible analysis software and a CpG assays-design software.