Anaspec has released the patent-pending Sensolyte 520 thiol quantitation kit: an ultra-sensitive fluorescent assay for the measurement of total thiol levels.
This kit contains a novel fluorogenic reagent that, upon reaction with thiol, releases fluorescence.
Fluorescence signals can be detected at the common green excitation/emission wavelengths of 490nm/520nm.
The assay has a linear range of 0.04mM to 160mM thiol.
Common contaminants, such as DMSO and Triton X-100, are well tolerated in the assay.
Autofluorescence interference from cellular components or from test compounds is minimal at the emission wavelength of 520nm.
