Thermo Fisher Scientific has unveiled products designed to enhance qualitative and quantitative proteomic workflows, enabling scientists to analyse, identify and quantify more proteins in less time.
Thermo Fisher's Pinpoint software is designed to simplify the transition from early-stage biomarker discovery to larger-scale, quantitative verification of putative biomarkers, as well as general quantitative proteomics.
It helps researchers develop and run intelligent SRM (ISRM) methods that maintain the accuracy and precision of targeted protein assays while increasing throughput.
This Pinpoint software is a full commercial release that includes support and documentation as well as the ability to process hundreds of raw files in a single analysis.
Isotopically labelled, non-radioactive peptides function as critical internal standards for protein quantitation experiments with mass spectrometry and nuclear magnetic resonance spectroscopy.
Thermo Fisher Scientific now offers multiple grades of heavy peptides, enabling researchers to match the precision and cost of the internal standard according to the assay development stage.
The Thermo Scientific Heavypeptide Aqua Ultimate kit is designed for absolute quantification.
The Heavypeptide Aqua Quantpro kit is a more affordable alternative when ultimate precision is not necessary.
The Heavypeptide Aqua Basic kit is designed for relative quantification with lyophilised samples.
The latest Thermo Scientific mass spectrometers offer improved performance for proteomics analyses.
The LTQ Velos features a dual-cell linear ion trap and advanced ion optics to provide a highly sensitive linear ion trap mass spectrometer.
The LTQ Orbitrap Velos combines Velos technology with the Orbitrap mass analyser and a higher-energy collisional dissociation (HCD) cell to deliver ultra-high resolution and accurate mass data.
The LTQ Velos and LTQ Orbitrap Velos instruments increase the number of proteins identified with high speed and mass accuracy.
The updated Thermo Scientific Proteome Discoverer 1.1 software complements these mass spectrometers to provide a platform for the analysis of qualitative and quantitative proteomics data.
Proteome Discoverer 1.1 software includes capabilities for relative quantitation via isobaric labelling, such as tandem mass tags (TMT), batch searching, automated processing via a daemon, improved handling of large datasets, support for Mudpit experiments and the ability to merge data from multiple experiments.
The software complements recent introductions of Thermo Scientific Sieve 1.2 software for label-free differential analysis of proteins, peptides and metabolites, as well as Thermo Scientific ProsightPC 2.0 software for high-throughput processing of accurate-mass MS/MS data.
Discovery data from the Proteome Discoverer 1.1 software can be imported directly into the Pinpoint software to accelerate the development of targeted quantitative experiments.
The Thermo Scientific Pierce Human In Vitro Glycoprotein Expression Kit is designed to improve post-translational modification of proteins by synthesizing and accurately glycosylating proteins at much higher efficiencies.
When glycosylation is not necessary, the Thermo Scientific Pierce Human In Vitro Protein Expression Kit is available.
