Anaspec has introduced Sensolyte SIRT1 assay kits in the green range (Ex/Em=490/520nm), which can be used for high-throughput screening of SIRT1 inhibitors/activators.
The assay kits are claimed to offer the industry's longest wavelength.
The kits - the Sensolyte 520 FRET SIRT1 assay kit and Sensolyte Green SIRT1 assay kit - are two-step, homogeneous assays that use a FRET substrate with 5-FAM/QXL 520 as the FRET pair in the former and a green fluorophore-containing substrate in the latter.
The long wavelength fluorescence of the green dyes employed in these two kits is less interfered by the autofluorescence of cell components and test compounds, therefore making these kits ultra-sensitive.
Histone deacetylases (HDACs) act as transcriptional repressors of genes catalysing the removal of acetyl groups from a e-N-acetyl lysine of histone.
Sirtuins comprise a unique class of nicotinamide adenine dinucleotide (NAD+)-dependent deacetylases (class III HDACs) that target multiple protein substrates to execute diverse biological functions.
Sirtuins catalyse a reaction that couples lysine deacetylation to NAD hydrolysis, yielding O-acetyl-ADP-ribose and nicotinamide.
Sirtuin 1 (SIRT1), the human homolog of yeast Sir2 (Silent Information Regulator 2), is the most studied of the seven members of sirtuin family.
SIRT1 have been implicated in several important cellular processes, including genomic stability and DNA repair, mediated apoptosis, adipogenesis, and aging.
