Anaspec has developed a novel solid-support AMC resin for facile solid-phase synthesis of peptide-AMCs.
N-Fmoc-7-aminocoumarin-4-acetic acid (Fmoc-Aca-OH) is attached to Wang resin as a linker.
After Fmoc removal, peptides are synthesised on the amino group of the linker using standard SPPS Fmoc chemistry, followed by cleavage with trifluoroacetic acid.
The crude peptide-7-aminocoumarin-4-acetic acid conjugates (peptide-ACAs) undergo facile decarboxylation under mild conditions to afford peptide-AMCs.
