Thermo Fisher Scientific has announced enhancements for its proteomics tools that are said to to facilitate breakthrough progress in quantitative and qualitative proteomics.
The products work in concert with other Thermo Fisher Scientific products, including reagents and consumables to create simpler, time-saving workflows.
These solutions will be displayed at the Salt Palace Convention Center Salons 250BCEF, on 24-26 May, 2010, during the ASMS Conference on Mass Spectrometry and Allied Topics in Salt Lake City, Utah.
Thermo Scientific Pinpoint software simplifies the transition from early-stage biomarker discovery to larger-scale, quantitative verification of putative biomarkers.
It allows researchers to leverage previously acquired discovery data from mass spectrometers, such as the Thermo Scientific LTQ Velos and LTQ Orbitrap Velos, to speed and simplify development of quantitative assays using intelligent SRM (iSRM).
iSRM, available on all Thermo Scientific TSQ triple-stage quadrupole instruments, increases the sensitivity, selectivity and throughput of targeted quantitation assays, allowing the simultaneous verification and quantification of thousands of targeted peptides in a single run.
A prototype version of Pinpoint software was distributed to over 300 proteomics researchers worldwide, who used it to increase productivity and further proteomics research.
Thermo Fisher Scientific incorporated their feedback into a commercially available version, with enhancements that are designed to optimise productivity, data quality and confidence in results.
The software now processes a large number of data files, enables researchers to incorporate information from global protein and peptide spectral databases, and has been enhanced to facilitate analysis of phosphopeptides and glycopeptides.
Proteome Discoverer software is a comprehensive platform for the analysis of quantitative and qualitative proteomics data.
It addresses the need for flexibility by offering a range of bio-software tools and customisable workflows for every imaginable proteomics experiment, according to the company.
Proteome Discoverer software has new capabilities that boost productivity and improve quantitative results.
It supports a range of stable isotope labelling with amino acids in cell-culture (SILAC) experiments to speed quantitation of protein expression levels from differentially treated cell populations.
It also offers enhanced capabilities for relative quantitation using isobaric mass tags, such as Thermo Scientific Mass Tags (TMT), with statistical analysis of results.
Flexible handling of peptide identification improves coverage and relative quantitation, and data processing following data collection can now be automated.
The Thermo Scientific Easy-nLC II system uses a split-free design to achieve stability and reproducibility.
It is designed exclusively for proteomics and provides nanoflow performance out of the box.
It is easily coupled with the Thermo Scientific ion trap, hybrid and triple quadrupole mass spectrometers.
The Easy-nLC II features an intuitive graphical user interface, touch-screen operation and remote diagnostics capability.
It is compact and includes an autosampler that provides reproducible low-nanoliter injections.
The Easy-nLC II includes new features designed to boost performance and productivity, including support for unattended analysis of 384-well plates; an integrated, refrigerated autosampler for improved sample stability; and intelligent flow control to speed equilibration and sample loading.
The EASY-nLC II provides 2D chromatographic capability to identify more proteins than conventional methods, without the increased complexity of added valves and pumps.
Isobaric mass tags such as the Thermo Scientific Tandem Mass Tag Reagents enable concurrent identification and multiplexed relative quantitation of multiple protein samples using tandem mass spectrometry.
The Thermo Scientific cysteine-reactive tandem mass tag (cysTMT) reagents can be used to perform two-plex or six-plex quantitation of cysteine-containing peptides.
To selectively analyse the cysteine-labelled peptides, the sample is enriched using the Thermo Scientific immobilised anti-TMT antibody resin.
Together, the cysTMT reagent and immobilized anti-TMT antibody resin reduce sample complexity and improve dynamic range, making it possible to identify a unique set of proteins.
The Thermo Scientific Pierce Fe-NTA Phosphopeptide Enrichment Kit allows fast, efficient enrichment of phosphorylated peptides.
Its spin columns can parallel process multiple protein digests in less than 30min for analysis by mass spectrometry.
High-binding capacity resin allows enrichment of up to 150 micrograms of phosphopeptides per column.
The kit contains detailed instructions and all necessary components to load, wash and elute phosphopeptides.
It is compatible with samples digested in solution or after in-gel digestion.
