Illumina has launched the Truseq SBS sequencing kit for the Genome Analyzer system, designed to offer longer reads and enhanced data quality in sequencing applications.
The Truseq SBS sequencing kit will enable high-quality paired end reads of 150 base pairs (bp), increase the output of high-quality base cells, and enhance throughput to greater than 95 Gigabases (Gb) of high-quality base pairs per run on the Illumina Genome Analyzer (GA) sequencing system.
Individual products within the family of Truseq reagents will be specifically formulated for each Illumina sequencing platform to best leverage the features of that system to provide high yield and data quality.
Gregory May, president and chief operating officer at the National Center for Genome Resources (NCGR), said: 'At NCGR, we're using Truseq SBS chemistry to routinely generate more than 85 per cent Q30 data for our 100bp runs, which is an improvement in both accuracy and output.
'In addition, we're now able to perform paired-end reads up to 150bp on the Genome Analyzer, which will help us both in our de novo sequencing studies as well as our ongoing agriculture sequencing projects,' he added.
Coupled with advances in algorithms, the Truseq SBS sequencing kit will enable to increase the yield of Q30 base calls and generate approximately 500 million perfect reads per 2x100bp run.
The yield of perfect reads is an important indicator of a system's overall quality and helps in mapping accuracy, yield and overall coverage of a genome.
Illumina expects to follow up the release of the Truseq SBS sequencing kit with Truseq Cluster Generation kits and Truseq Sample Preparation kits for both DNA and RNA applications on the GA later in 2010.
