Anaspec has highlighted its Hilyte Fluor dyes for labelling amine and thiol modified oligonucleotides from its collection of amine and thiol reactive six-isomeric dyes of fluoresceins and rhodamines.
Fluorescent dye-labelled oligos are widely used as primers for sequencing and PCR reactions, and also as probes for fluorescence in situ hybridisation.
Double-labelled oligos are used to produce fluorescence resonance energy transfer (FRET) for a number of applications such as real-time PCR assays and automated DNA sequencing.
Synthetic oligos can be modified with amine or thiol groups at their 5'-end, 3'-end or internally.
These amine and thiol incorporated oligos can then be labelled with amine and thiol reactive fluorophores, respectively.
N-hydroxysuccinimidyl esters (SE) and isothiocyanates of fluorescein and rhodamine derivatives are used for labelling amine-modified oligos.
Maleimides and iodoacetamides of fluorescein and rhodamine derivatives are used for labelling thiol-modified oligos.
Anaspec's proprietary Hilyte Fluor dyes are available in the SE and maleimide forms.
These dyes have fluorescence emission wavelengths spanning the full visible and near-infrared (NIR) spectrum.
Hilyte Fluor dyes are said to have properties that are superior to conventional dyes, with high-fluorescence quantum yields, large extinction coefficients, good photostability, low pH sensitivity and improved water solubility.
