Anaspec has released the mouse monoclonal Anti-MOG (1-125) for the advancement of MS research.
Anti-MOG monoclonal antibody was produced by immunising Balb/c mice with recombinant MOG (1-125) as antigen.
It is supplied as purified mouse IgG1, 250ul in 40mM MOPS (pH 7.5) with 50 per cent glycerol containing 0.1 per cent sodium azide and 0.1 per cent BSA.
Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin super-family and is expressed exclusively in the central nervous system (CNS).
MOG protein constitutes only 0.01-0.5 per cent of the CNS myelin proteins, has a mass of 27kDa and is located in the outermost layer of myelin sheath (1-6).
Mouse MOG is able to induce auto-antibody production and relapsing-remitting neurological disease, causing extensive plaque-like demyelination (1-4).
Autoantibody response to mouse MOG has been observed in induced experimental autoimmune encephalomyelitis (EAE) in C57/BL6, 129/Sv, B6 and SJL mice (3-4).
Anaspec has offered other MS research products, including MOG proteins, peptides, antibodies and assay kits.
The MOG proteins, with sequences corresponding to human, mouse and rat extracellular domain, together with a 6x His tag, were expressed in E coli and purified from urea denatured bacterial lysate.
The molecular weight found in all three recombinant proteins was 14.23kDa.
These proteins were shown to induce EAE in either mouse (for the human and mouse rMOGs) or rat (for rat rMOG).
Anaspec's MOG (35-55) peptide from mouse and rat sequence was shown to induce anti-MOG (35-55) autoantibody production in C57BL/6 mice at 100 per cent incidence (10/10); providing quantitative data that this peptide is suitable for in vivo anti-MOG (35-55) autoantibody study.
A cysteine-containing MOG (35-55) peptide induced polyclonal anti-MOG (35-55) antibody production in rabbits with high specificity, high affinity and high titer indicating its high purity, as well as suitability for use in peptide-coated ELISA and Western blot.
This mouse/rat MOG (35-55) peptide was used as a coating peptide to develop the industry's first anti-MOG (35-55) ELISA kit for EAE study.
The detection limit of the kit was determined to reach as low as 50pg of anti-MOG (35-55) auto-antibody in serum or cerebrospinal fluid.
Lot-to-lot variability of the MOG (35-55) was found to be minimal.
As for MOG Polyclonal Antibodies, one rabbit polyclonal antibodies towards the MOG extracellular domain raised against the human recombinant protein and the other against the mouse recombinant protein.
These Protein A affinity purified antibodies recognised a 27kDa band.
The Sensolyte Anti-Human MOG (1-125) IgG Quantitation ELISA Kit, Sensolyte Anti-Mouse MOG (1-125) and Sensolyte Anti-Rat MOG (1-125) IgG ELISA assay kits are for determining the amount of anti-human MOG (1-125), anti-mouse MOG (1-125) and anti-rat MOG (1-125) antibodies.
The improved Sensolyte Anti-MOG (35-55) IgG Quantitative ELISA Kit (mouse/rat) is optimised to detect anti-MOG (35-55) mouse/rat IgG.
Using strips pre-coated with MOG (35-55) peptide and pre-blocked with BSA, the amount of anti-MOG IgG subtypes in serum or cerebrospinal fluid can be determined by ELISA and the absorbance read at 450nm.
