Cultrex stem-cell-qualified basement membrane extract (BME), Pathclear is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumour.
The extract forms a reconstituted basement membrane mainly comprised of laminin, collagen IV, entactin and heparin sulphate proteoglycan.
Cultrex stem-cell-qualified BME provides an effective feeder-free surface for the attachment and maintenance of human embryonic stem cells in a pluripotent state, thereby enabling its use for growth promotion or for study of stem-cell differentiation.
This is demonstrated by the presence of Oct-4 and Nanog in H9 human embryonic stem cells grown on Cultrex stem-cell-qualified BME.
The Pathclear designation means that in addition to standard sterility, endotoxin and MAP testing, the BME is tested by PCR and is clear of 31 pathogens and viruses, including LDEV.
Each lot is rigorously qualified in biological performance assays.
