Anaspec is offering the anti-5-methylcytidine (Clone 33D3) monoclonal antibody for use in epigenetics research.
5-Methylcytidine is a modified base found in the DNA of plants and vertebrate.
DNA methylation is a post-replication process that is involved in the establishment of genomic imprinting, and the control of gene expression and differentiation.
Alterations in the DNA methylation pattern are associated with carcinogenesis.
A global DNA hypomethylation is often detected in tumour tissues and associated with local hypermethylation sites.
Since this antibody discriminates between the modified base 5-MeCyd and its normal counterpart cytosine, it is said to be a useful tool for gene promoter methylation analysis.
This antibody has been used to detect alterations in the urinary excretion of nucleosides from cancer patients, to visualise the distribution of methyl-rich regions along human chromosomes and to quantify in situ differences between normal and malignant cells from peripheral blood, as well as on tissue sections.
Anti-5-Methylcytidine antibody from Clone 33D3 can also be used in applications such as Methylated DNA Immunoprecipitation (MeDIP), MeDIP-on-chip assay, immunofluorescence, flow cytometry and immunohistochemistry.
The histopathological analysis of tissue samples can be performed without having to destroy cells through DNA-extraction procedures.
