Amsbio has introduced the ExpressArt mRNA amplification kit, suitable for overcoming the limitations of sample quality and quantity in zRT-PCR and array studies, even with degraded RNA sequences.
ExpressArt technology allows reproducible and precise analysis of challenging samples including Formalin-Fixed Paraffin-Embedded (FFPE), laser-captured micro dissections, needle biopsies and circulating tumor cells.
Based on a unique non-eberwine method using patented TRinucleotide primers and reagents, ExpressArt minimises loss of sequence associated with two-round amplification procedures and eliminates 3' bias in amplified products.
The resultant RNA produces accurate data on differential expression of low-copy-number genes that fail to give sufficient signal intensities using alternative methods, allowing direct comparison of data from a wide range of input sample sizes.
In addition using this exclusive primer technology, ExpressArt is able to amplify severely degraded RNA sequences, enabling accurate analysis of RNA with extremely poor RIN values.
An optimised ExpressArt kit is available that allows specific amplification of bacterial mRNA from any species without the need for additional procedures to deplete ribosome RNA.
ExpressArt's amplification technology also provides amino-allyl labeling modules and exclusive reagents for enhancing RNA isolations from very small and FFPE samples.
