AnaSpec's latest GFP antibodies are two monoclonals raised in mouse and a polyclonal raised in chicken, for applications such as monitoring gene expression and localising GFP-tagged proteins in vivo.
Additionally capable of recognising other green-fluorescent protein (GFP) mutant forms, the reactivity of these antibodies was confirmed by ELISA and specificity was confirmed by Western Blot using GFP.
GFP is a 27-kDa protein, which was originally cloned from Jellyfish cnidarians, Aequorea victoria.
It absorbs blue light (maximally at 395nm) and emits green light (peak at 509nm) without any requirement for exogenous substrates and cofactors.
These intrinsic fluorescence properties make GFP useful for cell biology research in applications such as monitoring gene expression and protein localisation of GFP-tagged proteins in vivo.
Other applications of GFP include assessment of protein-protein interactions through the yeast two-hybrid system and measurement of distance between proteins through fluorescence energy-transfer (FRET) protocols.
Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maximum when compared to the wild type GFP, making them useful for FACS, fluorescence microscopy, and double-labelling applications.
