Ludger now sells 50ug, 100ug and 250ug quantities of bovine fetuin, a major glycoprotein synthesised by hepatocytes and found in foetal calf serum, for use in glycoprofiling as a positive control.
The structure of bovine fetuin's carbohydrate portion of fetuin has been extensively studied and is regularly used as a positive control glycoprotein standard in glycoprofiling studies.
It exists in a variety of glycoforms containing bi-, tri- and tetra-antennary oligosaccharides with variable sialylation.
The N-glycans have been characterised at Ludger using a combination of glycoprofiling techniques, including charge separation by anion exchange HPLC followed by exoglycosidase sequencing with analysis by HILIC (amide) HPLC, thus identifying the N-glycan structures and their relative abundance.
This information will be of interest to glycoprofiling teams wishing to use fetuin as a positive control.
