The liquid-stable Haptoglobin kit from Randox provides a fully automated, highly sensitive and specific method for the quantitative determination of Haptoglobin in human serum.
The method is suitable for use on the RX series of bench top clinical analysers including the RX daytona and RX imola.
Haptoglobin is an acute phase protein made primarily in the liver.
Its main function is to bind free haemoglobin and remove it from circulation preventing damage to the kidneys and loss of iron following hemolysis.
Haptoglobin measurements are mainly used to diagnose haemolytic anaemia and to distinguish it from other types of anaemia.
In cases of haemolytic anaemia, Haptoglobin levels in the blood decrease significantly however low levels may also indicate red blood cell destruction due to sickle cell anaemia or Thalassemia.
As an acute phase reactant, Haptoglobin levels in the blood are significantly increased in response to infection, inflammation, burns, surgery and trauma, however, Haptoglobin is not generally used to diagnose or monitor these conditions.
The Randox Haptoglobin assay is based on an immunoturbidimetric method where Haptoglobin in the sample is diluted and reacted with specific antiserum yielding insoluble aggregates that can be measured turbidimetrically at 340nm.
A wide measuring range of 0.13g/l - 3.68g/l ensures the assay is capable of accurately measuring above and below the patient normal range while an extended onboard stability of 28 days minimises reagent waste.
It is also traceable to certified reference material, and has a within-run precision less than five per cent CV, total-run precision Randox's liquid-specific protein controls are stable for 30 days at 2-8C and are 100 per cent human in origin. Furthermore, three clinically significant levels are available with assayed target values and ranges for 27 different analytes including Haptoglobin. In addition to quality control, Randox also has a specific protein EQA programme available to provide customers with a complete quality-management solution. The bi-weekly RIQAS programme comprises 26 proteins and allows assessment of analytical performance in comparison to other laboratories using the same method/instrument.
