ATCC (American Type Culture Collection) has introduced the Universal Mycoplasma Detection Kit.
The kit brings together universal primers, optimised reagents and touchdown PCR to offer a highly sensitive and specific assay at an economical price.
Working with experienced gained in managing more than 3,600 cell lines, ATCC scientists designed the Universal Mycoplasma Detection Kit to provide fast results in an easy-to-use format and improve the overall level of cell-culture quality.
The ATCC Universal Mycoplasma Detection Kit provides all the components needed for an optimised PCR reaction, including buffers for cell lysis, sample lysis tubes, PCR mixes and primers.
Universal primers that are specific to the 16S rRNA coding region in the Mycoplasma genome are used with a thermostable DNA polymerase.
Touchdown PCR avoids amplification of nonspecific sequences and increases specificity to the extent that DNA originating from other sources, such as E coli and human, mouse, hamster, herring sperm, dog or rat genomic DNA, are not amplified.
In approximately three hours, the kit will detect as few as 10 genomes of M arginini.
The ATCC Kit is highly selective, recognising over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma, including the eight species most likely to contaminate cell cultures: M arginini, M fermentans, M hominis, M hyorhinis, M orale, M pirum, M salivarium and A laidlawi.
