Anaspec Eurogentec has introduced the Cherryexpress kit, which allows direct visualisation through the whole process of T7 protein expression, production and purification without special requirements.
A simple absorbance reading at 413nm gives specific and accurate calculation of the target protein concentration at each step.
The Cherryexpress kit provides easy quantification and easy-to-follow purification steps.
When using the Cherryexpress it is possible to quantify the protein concentration at any step, from protein production to the end of purification process.
A simple absorbance measurement at 413nm allows specific and accurate calculation of the target protein concentration.
The red colour constitutes a visual marker throughout the protein purification steps.
Concentration of the fusion protein can be determined easily by spectral measurement at 413nm, both before and after purification.
After purification, the tag can be cleaved using Enterokinase (a recognition site is inserted at the C-terminal end of the tag sequence).
The Cherryexpress system is also appropriate for rapid screening and optimisation of protein solubility.
The kit provides visual aid for 'live' estimation of the best conditions such as time and temperature.
There is no need to lyse cells to follow the purification process to determine if the protein is expressed or not.
Lack of solubility is a major problem when expressing recombinant protein in E coli.
However, when using the Cherryexpress vector, the Cherry tag (heme binding part of cytochrome, 11 kDa) is highly soluble and thus can increase the solubility of target proteins.
PSCherry1 and PSCherry3 vectors allow expression of protein with the Cherry tag at the N- or C- terminus, respectively.
The Cherry tag inserted in the Stabyexpress T7 vector gives multiple advantages such as T7 expression, plasmid stabilisation without antibiotics and protein visualisation.
