Qiagen has introduced the Investigator Quantiplex kit for DNA quantification in forensic samples, designed to quickly confirm that samples contain sufficient DNA for genetic fingerprinting analysis.
The kit quantifies the human DNA in the sample and establishes whether it contains inhibitors that may interfere with downstream applications.
DNA quantification with the Investigator Quantiplex kit is based on real-time PCR.
It provides accurate data in less than 50 minutes with the Rotor-Gene Q thermocycler, but is also compatible with other instruments.
Qiagen claims the kit provides faster results than any other kit on the market.
Reliable results are ensured by employing the appropriate target sequence and an integrated internal control that tests for successful amplification or PCR inhibition.
The overall sensitivity is less than 1pg/ul.
The procedure can also be automated on the Qiagility system for rapid, high-precision automated PCR set up.
The Investigator Quantiplex kit provides quick results and enables higher accuracy than found with total DNA quantification, Qiagen said.
External scientific partners validated the marker for the real-time PCR.
No copy-number variation has been detected between genders or across populations, making this marker a suitable target for DNA quantification.
The assay completes the forensic workflow offering at Qiagen from sample extraction solutions optimised for forensic samples, as well as advanced assay set-up and detection platforms tailored to different throughput and application needs in the laboratory.
