Anaspec, EGT Group, has introduced the Sensolyte Rh110 DPPIV assay kit, a long-wavelength fluorimetric assay kit for determining Dipeptidyl Peptidase IV (DPPIV) activity.
This kit contains a substrate that emits in the green range of 520nm when cleaved by DPPIV.
It provides a convenient assay for enzyme inhibitors and activators screening or for the continuous assay of enzyme activity.
Cleavage of the fluorogenic substrate by DPPIV generates the Rh110 (rhodamine 110) fluorophore, which has a bright green fluorescence.
Fluorescence can be monitored at excitation/emission=490/520nm.
The longer-wavelength spectra and higher extinction coefficient of Rh110 provide greater sensitivity and less interference from other reaction components.
Dipeptidyl Peptidase IV (DPPIV, DPP-4, CD26) is a cell surface serine protease, which cleaves Xaa-Pro or less efficiently Xaa-Ala dipeptides from the N-terminus.
DPPIV possesses an identical function to the T-cell surface antigen (CD26).
DPPIV is commonly found in mammalian tissues and is highly expressed in hepatocytes, pancreatic epithelial cells, intestinal epithelium, and kidney cortex.
DPPIV plays an important role in glucose homeostasis by proteolysis of incretins such as glucagon-like peptide-1 (GLP-1).
Due to its role in degradation of GLP-1, DPPIV is a therapeutic target for type 2 diabetes.
The SensolyteAMC DPPIV assay kit, which employs an AMC fluorogenic substrate, is also available from Anaspec.
This kit is optimised for screening of enzyme inhibitors.
This kit contains an AMC labelled substrate that has a high reactivity and low background.
Upon protease cleavage, this substrate generates the AMC fluorophore emitting bright blue fluorescence that can be monitored at excitation/emission=354/442nm.
Increase in AMC fluorescence is proportional to DPPIV activity.
