Chemists at Anaspec, Eurogentec Group, have developed a Wang resin conjugated with a pNA analog, 5-amino-2 nitrozenzoic acid (Anb5,2).
Colorimetric-based Peptide-p-nitroanilide conjugates (peptide-pNAs), with absorbance at approximately 408nm, have historically been and are still widely used substrates for the study of protease activity.
However, the preparation of peptide-pNA presents technical challenges.
The amino group of pNA has a low nucleophilic property due to the electron-withdrawing effect of the nitro group; poor solubility of a p-nitroanilide intermediate and coupling in solution phase by DCC, azide or active ester, techniques commonly used are not effective.
Chemists at Anaspec, Eurogentec Group, have developed a Wang resin conjugated with a pNA analog, 5-amino-2-nitrozenzoic acid (Anb5,2).
This support for facile solid-phase pNA-like peptide substrate syntheses was developed based in part on a paper by Hojo et al, in which they described the introduction of Anb5,2 to a p-methylbenzhydrylamine (MB) resin.
The Wang preloaded resin is a useful tool for the synthesis of peptide with a C-terminal pNA-analog, Anb.
Anaspec also presented a poster at the recent ABRF meeting in San Antonio, Texas, entitled 'Facile Solid-Phase Synthesis of Peptide-7-p-Nitroanilide (pNA) Analog Containing Conjugates Using a Novel Wang or Rink Amide Resin.'
