Oligonucleotide synthesis specialist Integrated DNA Technologies (IDT) offers a range of custom assays, primers and probes for qPCR applications.
Primetime qPCR assays are available in three sizes (mini, standard and XL) and provide the forward and reverse primers, as well as a hydrolysis probe in a single tube.
Primers and probes are also available separately, including the double-quenched probe, which increases accuracy and sensitivity through the incorporation of an internal Zen quencher.
As such, IDT said users are provided with an option to meet any experimental requirements.
Primetime qPCR assays allow users to select from multiple dye-quencher combinations, as well as define primer probe ratios to optimise experimental setup.
Each oligo undergoes 100 per cent QC by mass spectrometry and is shipped in two to four days of order.
IDT also offers Primetime mini qPCR probes, which are said to be suitable for gene expression screens.
Mini probes are offered with a 5' FAM and the option of a 3' IBFQ quencher alone, or in combination with the internal Zen quencher.
Users can also buy Express probes, which are shipped in one working day, making them suitable for time-sensitive laboratories.
IDT also offers Primetime pre-designed assays that are guaranteed by the company to work for human, mouse and rat.
Users are said to routinely experience increased sensitivity and higher qPCR when using these assays.
The design process uses the most current, clean and complete target sequence information available and stresses accurate Tm prediction and protection against off-target amplification.
The ordering system contains assays designed to target exon boundaries for every human, mouse and rat transcript in the NCBI database.
