Anaspec's Sensolyte 520 TACE assay kit is claimed to offer fluorescence detection at the industry's longest wavelengths, for the inhibitor screening of Tumour Necrosis Factor alpha (TNF-a).
With superior fluorescence quantum yield and longer emission wavelength, this kit shows less autofluorescence interference from test compounds and cellular components, and provides better assay sensitivity than comparable kits using shorter-wavelength FRET substrates.
The Sensolyte 520 TACE activity assay kit uses a FRET peptide substrate for the continuous measurement of enzyme activity.
The FRET pair is composed of 5-FAM (5-carboxyfluorescein) and QXL 520, one of Anaspec's proprietary fluorescence quenchers.
In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL 520.
Upon cleavage of the FRET peptide by the active enzyme, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490/520Nm.
The kit can be used to detect the activity of TACE or screen for TACE inhibitors.
Tumour Necrosis Factor alpha (TNF-a) Converting Enzyme (TACE), also called ADAM17 or a-Secretase, belongs to the ADAM (A Disintegrin and Metalloprotease) family of proteins involved in myogenesis, neurogenesis and fertilisation through the ectodomain shedding of cell surface proteins.
