AnaSpec has announced the release of an ultrasensitive ELISA assay for quantitatinga-synuclein.
The SensoLyte a-Synuclein Quantitative ELISA Kit (Human/Mouse/Rat) provides a convenient and quantitative assay for determining a-Synuclein amount in cell and tissue lysate as well as in body fluids.
The one-step, mix-and-read assay requires less time to run.
HRP conjugated detection antibody is added simultaneously with the samples and standards during the assay, eliminating extra incubation and washing steps.
a-Synuclein is a major component of Lewy bodies found in affected neurons of patients with Parkinson's disease.1-4 This protein has a mass of 14.5 kDa (140 amino acids long) and consists of a conserved degenerative amino-terminal domain and an acidic carboxyl-terminal that has a higher sequence divergence.1-4 a-Synuclein is predominantly expressed in brain, specifically in cerebellum, thalamus, neocortex, hippocampus, and striatum regions.1-2 Other tissues also express a-Synuclein but at very low levels.1-4 The physiological role of a-Synuclein is not well understood.
However, the presence of imperfect KTKEGV lipid interacting repeats suggests that it may be involved in synaptic vesicle homeostasis.
