SPRIselect claims to speed and simplify genomic DNA size selection for fragment library preparation.
SPRIselect from Beckman Coulter Life Sciences is based on SPRI (Solid Phase Reversible Immobilisation)-based chemistry.
Following shearing, the fragment library construction process requires size selection to produce uniform distribution of fragments.
SPRIselect allows size distribution to be adjusted between 150 and 800 base pairs to suit the application and sequencing platform.
The process can be performed manually or automated for high throughput in 96-well plates on platforms such as Beckman Coulter Life Sciences’ Biomek Workstations.
Features include:
- Easily tune size ranges between 150 to 800 bp
- Accurate DNA sizing without gels or special instrumentation
- Optimizs using left or right side size selection only or run both (double) for tighter sizing
- Combine efficient mag bead technology with automation for increased productivity
- Achieve consistent results, reproducible sizing between runs and reagent lots
- Leverage proprietary technology already the gold standard for purification steps in Next Gen Sequencing (NGS) sample prep
SPRIselect reagent kits are available in 5, 60 and 450 mL volumes, enable rapid and consistent size selection, and come with guidelines to assist users in customising protocols.
Gel cartridges, chips and additional instruments are not needed. Samples should be fragmented, double-stranded DNA of 50mL or greater, dissolved in molecular biology grade water or such standard buffer solutions as Tris or TE.
SPRIselect is ideal for use in most common next generation applications, including paired-end, single-end, targeted, ChiP and RNA sequencing, and for use with samples to be run on such platforms as Illumina’s MiSeq, HiSeq and Genome Analyzer IIx; Roche’s 454 GS FLX and GS Junior; and Life Technologies’ SOLiD series and Ion Torrent PGM.