Rapidly developing epigenetics
30 Jul 2013
Porvair Sciences has announced that its 96-well ChromaTrap technology is capable of achieving reproducibility in screening multiple epigenetic targets.
Currently, researchers are elucidating the principles governing gene regulation through the in vivo dynamic binding of proteins to DNA.
Significant to this is the need to analyse large numbers of high and low abundant regulatory mechanisms to map protein-DNA interactions following pathogen and disease stimulus.
Chromatin immunoprecipitation (ChIP) assays are used to study these associations but can be limited to large sample numbers, sample losses and low signal to noise which generates poor reproducibility.
The Chromatrap 96 Pro-A system has been used to simultaneously bind efficiency and occupancy of common epigenetic marks on three gene targets in three human chromatin samples and then analysed using qPCR.
Porvair’s application note details the methodology used and offers various data interpretation and statistical analysis.
To download the application note, please click on the link below.
