New application for quantitative end-point RT-PCR gene expression measurement using an off-the-shelf bioanalyser and standardised reagents
The Agilent 2100 bioanalyser can be used in combination with standardised RT (Start)-PCR reagents from Gene Express National Enterprises (Gene) to obtain highly reproducible, standardised gene expression measurement with RT-PCR.
The internal standards used in Start-PCR control for variable loading of PCR products into electrophoresis channel, and allow quantitative gene expression measurement at the endpoint of PCR, without real-time analysis.
Gene expression measurement of 15 genes was more reproducible with the Agilent 2100 bioanalyser than with the ABI Prism 310 Genetic Analyser or agarose gel electrophoresis methods, says Agilent.
For further information, request application note 'Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 bioanalyser and standardised RT-PCR," publication number 5988-3674EN.
This application note is available without charge from any Agilent sales office or its website (see above).
