RNA isolation kit improves genomic DNA removal up to a thousand times without using DNase kit to aid researchers in microarray and PCR experiments
Agilent Technologies has introduced a new RNA isolation kit designed to remove up to 1000 times more genomic DNA than competing products.
The kit does not require the use of DNase enzyme, which is commonly used to remove genomic DNA but can compromise the quality of RNA.
To scientists using RNA in their experiments, genomic DNA is a significant contaminant that interferes with and affects data reliability.
Pharmaceutical, biotech, agbio, and academic researchers can use this kit to isolate highly purified, total RNA from tissues and cells for use in microarray-based gene expression, real-time PCR experiments, and other applications.
PCR is a widely used process for copying and quantifying DNA or RNA.
The Agilent Total RNA Isolation Mini Kit uses a pre-filtration column that reliably removes genomic DNA.
It can reduce genomic DNA contamination to less than one femtogram per nanogram of isolated RNA in selected tissues.
This minimises experimental failure in microarray and real-time PCR experiments due to impure RNA, and provides better data for these critical applications.
This method also saves researchers the time and cost of doing the DNase treatment.
"This is one of several breakthrough bioreagent products that Agilent is developing for gene expression and proteomics solutions, said Fran DiNuzzo, vice president and general manager of Agilent's bioreagents, consumables, and services business. "To continually improve the quality of gene expression results for a scientist, we are addressing every element of the workflow, from sample preparation to microarrays, scanners and data analysis software, optimising every step in the researcher's workflow."
