Scientists at Ceph turned to Applied Biosystems when they needed high throughput equipment to analyse differentially expressed genes in hepatocellular tumours
A group of Inserm scientists at the Centre d'Etude du Polymorphisme Humain (Ceph) in Paris turned to Applied Biosystems when they needed high throughput equipment to analyse differentially expressed genes in hepatocellular tumours.
Project leader Jessica Zucman-Rossi explained: "The aim of our research is to understand the molecular events and characterise the pathways involved with the development of hepatocellular carcinomas and, from there, to identify new diagnostic and prognostic markers.
"Following the identification of a number of differentially expressed genes, we use Applied Biosystems's Assays-on-Demand kits to analyse their expression in a series of 100 hepatocellular carcinomas, benign tumours and corresponding non-tumour liver tissues.
"It was important for us to find a robust system that could cope with the high workload this involved and would allow us to standardise our procedures.
"The Applied Biosystems TaqMan low density array looked very promising in both of these respects, and we were impressed that Applied Biosystems offered such a large range of preloaded and validated assays that could be shipped to us quickly.
"We have found the quality of the gene database and annotations to be very high and like the fact that the human assays can be complemented with mouse assay sets if required. "The Applied Biosystems application support team helped us to design the Assays-on-Demand product analyses for our tumour series and the experimental protocols to go with it.
"They were also on hand to help us validate and interpret the first results we obtained."
