Automated two-dimensional protein fractionation system enhances proteome analysis, resolving the thousands of proteins present in cell lysates
Beckman Coulter introduces the ProteomeLab PF 2D, an automated, two-dimensional fractionation system for high-resolution analysis of complex protein mixtures.
Part of the company's new ProteomeLab family of products, the PF 2D produces results in just hours.
The system effectively resolves the thousands of proteins present in cell lysates, addressing a key challenge in today's proteomics research.
In the first dimension, chromatofocusing separates the proteins according to their pI, providing information that can be related to traditional isoelectric focusing techniques.
Fractions from the first dimension are collected and automatically injected into a second dimension, which separates on the basis of hydrophobicity. ProteomeLab PF 2D software displays both one- and two-dimensional maps of the expressed protein profiles in easy-to-read formats. This 'in solution' approach delivers liquid fractions for further study.
The PF 2D system addresses many of the problems associated with traditional proteomics research, such as detection of low abundance proteins, run-to-run reproducibility, quantitation, detection of membrane or hydrophobic proteins, detection of basic proteins and detection of very low and very high molecular weight proteins.
The automated system also requires less time and manpower than traditional, labour-intensive techniques.