These specially designed and functionally tested constructs can reduce TF gene expression in vivo by as much as 90% allowing researchers to study an experimental system with little TF activity
Cambridge BioScience has introduced new TranSilent siRNA vectors from Panomics for transcription factor (TF) knockdown.
These specially designed and functionally tested constructs can reduce TF gene expression in vivo by as much as 90% allowing researchers to study an experimental system with little TF activity.
21-25 nucleotides in length, small interfering RNAs (siRNAs) are intermediates of the RNA interference pathway which causes sequence-specific degradation of mRNA and silences gene expression.
TranSilent vectors contain a region encoding an RNA sequence that is post-transcriptionally converted into an siRNA-like transcript.
Each TranSilent siRNA vector targets a specific TF mRNA sequence and a range of vectors is available targeting: c-Fos, c-Myb, GR, p53, NfkB p65, and PPAR. Alternatively, your own insert can be cloned into the TranSilent control siRNA vector.
The vectors can be used in a wide variety of experimental models including primary cell cultures and live animals and allow researchers to monitor a system with substantially reduced TF activity.
TF knockdown is achieved through a simple transfection method with high stability.
The promoter region ensures constant expression, unlike transient methods such as RNA microinjection.
The vector becomes a permanent fixture in the cell line making multigenerational studies of gene knockdown possible.
